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KMID : 1098420090170060397
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Korean Journal of Medicinal Crop Science
2009 Volume.17 No. 6 p.397 ~ p.406
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High Pressure Extraction Process of Low Quality Fresh Ginseng for Enhancing Anticancer Activities
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Ha Ji-Hye
Kim Young
Kim Seung-Seop
Jeong Myeong-Hoon
Jeong Heon-Sang
Jung Jae-Hyun
Yu Kwang-Wan
Lee Hyeon-Yong
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Abstract
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The low quality fresh ginseng was extracted by water at 80℃ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at 100℃ in adding the maximum concentration of 1.0 ㎎/㎖. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding 1.0 ㎎/㎖ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 ㎎/㎏/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-Rg2, Rg3, Rh1 and Rh2 on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.
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KEYWORD
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Low Quality Fresh Ginseng, High Pressure Extraction Process, Ginsenoside, Anticancer Activity
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